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Use a dosage of 1 mcg/kg to 10 mcg/kg body weight for subcutaneous or intramuscular injection

Immortalized BMDMs were maintained in RPMI 1640 with 2 mM L-glutamine, 1 penicillinstreptomycin (GenClone), 10% (v/v) heat-inactivated FBS (R&D Systems) and 20% conditioned medium

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doi:10.1007/s11696-021-01826-1